2017-07-30 17:44:122025-05-15 17:09:25
locus
BSU00830
BSU_00830
geneLength
462
465
outlinks
bsu
BSU00830
BSU_00830
Gene
Coordinates
101,449 → 101,913
101,449 101,913
The protein
Protein family
ctsR family (according to Swiss-Prot)
CtsR family (single member, according to UniProt)
The protein
Structure
[PDB|3H0D] (complex with a 26bp DNA duplex, from ''Geobacillus stearothermophilus'') [Pubmed|19498169]
[PDB|3H0D] (complex with a 26bp DNA duplex, from ''Geobacillus stearothermophilus'') [Pubmed|19498169]
[PDB|6FH4] (C-terminal domain bound to phosphoarginine) [pubmed|30962626]
The protein
additional information
subject to Clp-dependent proteolysis upon glucose starvation [pubmed|17981983]
subject to Clp-dependent proteolysis upon glucose starvation [pubmed|17981983]
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000017 PRODORIC2 database]
Biological materials
Mutant
MGNA-B928 (yacG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1927 NBRP B. subtilis, Japan]
''ctsR::aphA3'' availbale in [SW|Ulf Gerth]'s lab
BKE00830 (''[[gene|ctsR]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
GP3202([[gene|ctsR]]::''ermC''), available in [SW|Jörg Stülke]'s lab
MGNA-B928 (yacG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1927 NBRP B. subtilis, Japan]
''ctsR::aphA3'' availbale in [SW|Ulf Gerth]'s lab
BKE00830 (''[[gene|ctsR]]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
BKE00830 ([[gene|ctsR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCAACCCCCTCCTTTAC, downstream forward: _UP4_AAATTAAAATAAGCGGGTGA
BKK00830 ([[gene|ctsR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCAACCCCCTCCTTTAC, downstream forward: _UP4_AAATTAAAATAAGCGGGTGA
Biological materials
Expression vector
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab
References
Reviews
References
Original Publications
Gene
Phenotypes of a mutant
reduced expression of [[gene|trxB]], due to reduced [[protein|Spx]] activity [pubmed|30962353]
The protein
Paralogous protein(s)
[[this]]
Expression and Regulation
additional information
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [SW|Ulf Gerth]'s lab